Supplementary Materials

The PDF file includes:

• Fig. S1. Comparison of conventional pathology and CytoPAN.
• Fig. S2. CytoPAN readouts for comixtures of benign and malignant cells.
• Fig. S3. Decision tree algorithm to classify cell populations.
• Fig. S4. Selection of optimal fixation methods for FNA specimen.
• Fig. S5. Selection of optimal permeabilization methods.
• Fig. S6. Comparison of direct staining (one-step) and indirect staining (two-step).
• Fig. S7. Stability test for lyophilized antibodies.
• Fig. S8. Bleed-through analyses and signal deconvolution.
• Fig. S9. CytoPAN user interface.
• Fig. S10. System characterization.
• Fig. S11. Comparison of CytoPAN and flow cytometry for different cell lines.
• Fig. S12. Comparison of CytoPAN and flow cytometry for repeat FNA from mice bearing four different breast tumor types.
• Fig. S13. Statistical analyses to determine cutoffs.
• Fig. S14. Quad as a cancer marker.
• Table S1. Pairs of primary and fluorophore-conjugated, species-specific secondary antibodies used in the study.
• Table S2. Cost of CytoPAN device.
• Table S3. Antibody list and cost for human samples.
• Table S4. Summary of patient cohort.

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Other Supplementary Material for this manuscript includes the following:

• Data file S1 (Microsoft Excel format).