Supplementary Materials

The PDF file includes:

  • Materials and methods
  • Fig. S1. Overview of the human and mouse studies included in this investigation.
  • Fig. S2. Characteristics of male and female FERKO mice.
  • Fig. S3. Expression analyses performed on adipose tissue samples from men participating in the MyoGlu studies.
  • Fig. S4. The impact of Esr1 loss and gain of expression on parkin and p53 cellular localization.
  • Fig. S5. Mitochondrial membrane potential in 3T3L1 adipocytes in the presence or absence of Esr1.
  • Fig. S6. ERα protein expression in gonadal and inguinal adipose tissue of BAT-specific ERα KO mice (ERαKOBAT).
  • Fig. S7. LC3B expression is reduced in BAT from ERαKOBAT mice.
  • Fig. S8. Schematic overview of the mechanisms by which ERα controls adipocyte metabolism and adiposity.
  • Table S1. Genes significantly correlated with ESR1/Esr1 and overlapping between the METSIM, MyoGlu, and HMDP studies.
  • Table S2. Genes associated with Esr1 in gWAT from male and female C57BL/6J mice.
  • Table S3. Mitochondrial-associated genes differentially expressed in gWAT from female FERKO versus Controlf/f mice.
  • Table S4. Mouse strains studied from the UCLA HMDP.
  • Table S5. qPCR primers used to assess gene expression in adipose tissue from WT C57BL/6J, Esr1 Controlf/f, FERKO, and ERαKOBAT mice.
  • Table S6. qPCR primers used for ChIP studies to assess the binding of ERα to the Polg1 proximal promoter in 3T3L1 adipocytes.
  • Legends for data files S1 to S6

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Raw data from studies of human tissues, cells, and genetically engineered mice.
  • Data file S2 (Microsoft Excel format). Raw data of ESR1-correlated genes in subcutaneous fat from men participating in the METSIM study.
  • Data file S3 (Microsoft Excel format). Raw data of ESR1-correlated genes in subcutaneous fat from men participating in the MyoGlu study.
  • Data file S4 (Microsoft Excel format). Raw data of Esr1-correlated genes in gonadal fat from male mice of the UCLA HMDP.
  • Data file S5 (Microsoft Excel format). Raw data of Esr1-correlated genes in gonadal fat from female mice of the UCLA HMDP.
  • Data file S6 (Microsoft Excel format). GO of microarray data performed on gonadal fat from female FERKO and Controlf/f mice.