Supplementary Materials

The PDF file includes:

• Materials and Methods
• Fig. S1. Design of the study.
• Fig. S2. Comparison of the RT-LAMP assay with CT values from RT-qPCR using primer sets E-Sarbeco and N-Sarbeco.
• Fig. S3. Comparison of the RT-LAMP assay using primer set 1a-A with CT values from RT-qPCR using primer set E-Sarbeco.
• Fig. S4. Analysis of LAMP-sequencing reads.
• Fig. S5. Sample classification with LAMP-sequencing.
• Fig. S6. RNA stability and detection limit of the RT-LAMP assay in pharyngeal swab specimens with IVT RNA.
• Fig. S7. Swab–to–RT-LAMP assay titration of positive COVID-19 specimens.
• Table S1. Sequences of primers and amplicons used in this study.
• Table S2. Sensitivity and specificity of the RT-LAMP test from Fig. 3B.
• Table S3. Sensitivity and specificity of the RT-LAMP test from Fig. 5B.
• Legend for table S4
• Table S5. Overview of time requirements for various sample handling steps.

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Other Supplementary Material for this manuscript includes the following:

• Table S4 (Microsoft Excel format). Primers used for LAMP-sequencing.
• Data file S1 (Microsoft Excel format). Raw data for figs. S6 and S7.