Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. CX3CR1high macrophages express CCR2 at high amounts.
  • Fig. S2. VAT macrophage proportions gradually decrease after birth.
  • Fig. S3. CX3CR1low CCR2low VAT-resident macrophages have a unique morphology compared to CX3CR1high CCR2high VAT monocyte-derived macrophages and monocytes.
  • Fig. S4. CX3CR1high CCR2high VAT monocyte-derived macrophages are proinflammatory.
  • Fig. S5. VAT and cardiac-resident macrophages have common canonical pathways.
  • Fig. S6. CX3CR1low CCR2low VAT-resident macrophages express a unique transcriptional profile compared to other tissue-resident macrophages.
  • Fig. S7. VAT-resident macrophages are predicted to express genes involved in lipid metabolism and insulin sensitivity.
  • Fig. S8. Post-MI insulin resistance is not accompanied by increased contents of cortisol and catecholamine, lipolysis, or heart failure.
  • Fig. S9. MI increases the number of circulating myeloid cells.
  • Fig. S10. Enrichment of genes involved in cellular apoptosis and necrosis in VAT-resident macrophages in C57BL/6 mice on day 7 after MI.
  • Fig. S11. Depletion of VAT-resident macrophage exacerbates glucose intolerance on day 7 after MI.
  • Fig. S12. MI alters hepatic metabolism.
  • Fig. S13. Csf1-deficient mice have reduced number of VAT-resident macrophages.
  • Fig. S14. Csf1 supplementation in Apoe−/− mice after MI decreases atherosclerotic plaque inflammation without affecting atherosclerotic plaque size.
  • Fig. S15. MI-induced insulin resistance is unique compared to other causes of insulin resistance.
  • Fig. S16. Schematic depicting potential mechanisms of MI-induced insulin resistance.
  • Table S1. Demographics of patients with STEMI.
  • Table S2. The primer sequences for the genes used in the qPCR experiments.
  • Legends for movies S1 and S2

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). MI decreases the frequency of VAT-resident macrophages.
  • Movie S2 (.mp4 format). MI decreases the frequency of VAT-resident macrophages.
  • Data file S1 (Microsoft Excel format). Raw data used in figures.