Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Culture and characterization of PTCs.
  • Fig. S2. Cellular characterization of PTCs.
  • Fig. S3. Genomic comparison of PTCs with the original tumors.
  • Fig. S4. Drug and genomic consistency of PTCs in different wells.
  • Fig. S5. PTCs recapitulating the treatment responses of patients with GC and CRC.
  • Fig. S6. Characterization of breast cancer PTCs and comparison of PTC cell viability and the treatment responses of patients.
  • Fig. S7. PTCs recapitulating the treatment responses of patients with triple-negative breast cancer.
  • Fig. S8. PTCs recapitulating the treatment responses of patients with luminal A or B breast cancers.
  • Fig. S9. PTCs recapitulating the treatment responses of patients with HER2+ breast cancer.
  • Fig. S10. Generation of PTCs from the ascites of patient CS-G-907 for drug testing.
  • Table S1. Summary of compounds tested on CRC or GC PTC media.
  • Table S2. Efficacy and concentrations of drugs used in this study.
  • Table S3. Gastrointestinal PTC growth medium.
  • Table S4. Breast cancer PTC growth medium.
  • Table S5. The primary antibodies used in immunohistochemistry and immunofluorescence assays.
  • Table S6. A cancer panel targeting 105 cancer-related genes.
  • References (4382)

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Fold change of 963 significant differentially expressed genes.
  • Data file S2 (Microsoft Excel format). All detected mutations between tumors and PTCs.
  • Data file S3 (Microsoft Excel format). The overlaps of mutations between tumors and PTCs.
  • Data file S4 (Microsoft Excel format). Clinicopathological data for patients with CRC or GC.
  • Data file S5 (Microsoft Excel format). Clinicopathological data for patients with breast cancer.
  • Data file S6 (Microsoft Excel format). Original data for all the PTC drug tests.
  • Data file S7 (Microsoft Excel format). BIC-seq2 segmentations.
  • Data file S8 (Microsoft Excel format). CNV correlations.
  • Movie S1 (.avi format). CRC self-assembly into PTCs.