Supplementary Materials

The PDF file includes:

  • Methods
  • Fig. S1. Base editing outcomes from different CBE and sgRNA combinations.
  • Fig. S2. Anc80-Cbh-GFP AAV transduction in IHCs and OHCs in wild-type mice.
  • Fig. S3. Base editing outcomes from mRNA of treated and untreated Baringo cochlea.
  • Fig. S4. Base editing at on-target and off-target genomic DNA sites identified by CIRCLE-seq using Cas9 + sgRNA1.
  • Fig. S5. Transduction currents from IHCs and OHCs of Tmc1Y182C/Y182C;Tmc2+/+ and Tmc1Y182C/Y182C;Tmc2/ mice at different time points.
  • Fig. S6. Hair cell morphology in the organ of Corti from Tmc1Y182C/Y182C;Tmc2+/+ mice with and without treatment with dual AAV-AID-BE3.9max + sgRNA1.
  • Fig. S7. Hair bundle morphology in the basal turn of the organ of Corti from Tmc1Y182C/Y182C;Tmc2+/+ mice with and without treatment with dual AAV-AID-BE3.9max + sgRNA1.
  • Fig. S8. Auditory function and correlation of ABR thresholds with surviving hair cells in Baringo mice at 4 and 6 weeks.
  • Table S1. P value calculation on ABR and DPOAE data from untreated wild-type mice and wild-type mice treated with dual AAV encoding AID-BE3.9max + sgRNA1.
  • Table S2. P value calculation on ABR data (5.6 to 16 kHz) from Baringo mice treated with dual AAV encoding AID-BE3.9max + sgRNA1 that showed ABR responses and untreated Baringo mice.
  • Table S3. Primers used for HTS.
  • Table S4. CRISPResso2 output for base editing at the target locus.
  • Table S5. List of base editing targets to correct known pathogenic point mutations in TMC1.
  • References (67, 68)

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Single data points and exact P values.