Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. FOXP3 TALENs catalyze efficient FOXP3 disruption and initiate seamless recombination of donor template.
  • Fig. S2. Purity of primary human tTregs and Teffs used in immunophenotyping assays.
  • Fig. S3. Human edTregs derived from CD4+ T cells exhibit increased sensitivity to IL-2.
  • Fig. S4. T cell sample preparation for RNAseq analysis.
  • Fig. S5. Transcriptome of Teff compared with tTreg and edTreg by RNAseq.
  • Fig. S6. The TCR repertoire after FOXP3 editing is highly polyclonal and similar to mock-edited T cells.
  • Fig. S7. Enrichment of edTreg using clinically relevant selection markers.
  • Fig. S8. edTregs, but not Teffs or mock-edited T cells, are nonpathogenic in a xeno-GvHD mouse model.
  • Fig. S9. Tissue histopathology studies in the xeno-GvHD mouse model.
  • Fig. S10. Body weight and GvHD scores of mice in the xeno-GvHD model.
  • Fig. S11. The phenotype and cytokine production of GFP cells in the xeno-GvHD model.
  • Fig. S12. edTregs generated using the CRISPR-Cas9 platform express Treg markers and promote immunosuppression in vivo.
  • Fig. S13. Design of TALENs targeting mouse Foxp3.
  • Fig. S14. EAE scores of mice with adoptive edTreg transfer and EAE induction.
  • Fig. S15. Antigen-specific human edTregs express Treg markers and are immunosuppressive in vitro.
  • Fig. S16. Antigen-specific edTregs exhibit antigen-specific and bystander suppression of Teff proliferation and cytokine generation.
  • Table S1. Rates of on- and off-target indels by human FOXP3 TALEN pairs.
  • Table S2. Top-ranked in silico predicted off-target indels of human FOXP3 Cas9 RNP.
  • Table S3. Oligonucleotide sequences.
  • Table S4. Flow cytometry antibodies.
  • References (6271)

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