Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. EZH2 is highly expressed in brain metastases and promotes brain metastases in a methyltransferase-independent manner.
  • Fig. S2. Nuclear Src binds to and phosphorylates EZH2 at Y696, reducing H3K27 trimethylation.
  • Fig. S3. pY696-EZH2 drives brain metastasis with cytokine reprogramming but has little effect on cancer cell growth and invasion in vitro.
  • Fig. S4. G-CSF recruits Arg1+/PD-L1+ neutrophils into the brains of mice bearing brain metastases.
  • Fig. S5. c-Jun regulates CSF3 and IL1A expression.
  • Fig. S6. pY696-EZH2 binds to and cooperates with RNA Pol II to up-regulate c-JUN transcription.
  • Fig. S7. pY696-EZH2 is associated with pY416-Src, pS73-c-Jun, and Ki-67 staining in brain metastases of patients with breast cancer.
  • Fig. S8. ICB combined with saracatinib impedes brain metastasis in mice.
  • Fig. S9. Src-induced pY696-EZH2 interacts with RNA Pol II to recruit immunosuppressive neutrophils that enhance brain metastasis.
  • References (8083)

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Preprocessed A375 complementary DNA microarray data.
  • Data file S2 (Microsoft Excel format). The reference table of cell lines and mouse groups.
  • Data file S3 (Microsoft Excel format). Original data for graphs.
  • Data file S4 (Microsoft Excel format). Gene dataset results from ChIP sequencing analysis.