Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Experimental design and sample sizes.
  • Fig. S2. CPS study profile and sample flow chart.
  • Fig. S3. RTS,S study profile and sample flow chart.
  • Fig. S4. PCA of CPS gene expression data.
  • Fig. S5. Heatmaps and unsupervised hierarchical clustering in gene expression after cell stimulation for all CPS immunized volunteers.
  • Fig. S6. PCA of RTS,S/AS01E gene expression data.
  • Fig. S7. Heatmaps and unsupervised hierarchical clustering in gene expression after cell stimulation for all RTS,S and comparator vaccinees.
  • Fig. S8. Frequencies of leukocyte subsets after resting of PBMCs.
  • Table S1. Predictive models obtained by artificial neural networks.
  • Table S2. Validation qRT-PCR values and prediction results for the selected CPS preimmunization signature of protection.
  • Table S3. Validation qRT-PCR values and prediction results for the selected CPS postimmunization signature of protection.
  • Table S4. Validation qRT-PCR values and prediction results for the selected RTS,S/AS01E postimmunization signature of protection.
  • References (98133)

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (.zip format). Gene lists differential gene expression CPS immunogenicity and protection.
  • Data file S2 (Microsoft Excel format). Gene list correlations PfRBC FC and CPS outcomes and frequencies of gene ontology biological processes terms and BTMs of genes correlated with CPS outcomes.
  • Data file S3 (.zip format). Gene lists differential gene expression RTS,S/AS01E immunogenicity and protection.
  • Data file S4 (Microsoft Excel format). Summary of GSEA and differential gene expression results.
  • Data file S5 (Microsoft Excel format). Summary of GSEA and WGCNA results.
  • Data file S6 (Microsoft Excel format). Selected genes for validation for CPS and RTS,S/AS01E.
  • Data file S7 (Microsoft Excel format). Postvalidation signatures of protection with accuracy data for CPS and RTS,S/AS01.
  • Data file S8 (Microsoft Excel format). CSP 15-mer peptides, corresponding to CSP region of RTS,S and predicted CD4+ and CD8+ T cell epitopes.
  • Data file S9 (Microsoft Excel format). Comparisons and limma contrasts.
  • Data file S10 (Microsoft Excel format). Gene list for validation.