Supplementary Materials

The PDF file includes:

  • Fig. S1. Time limit of protection.
  • Fig. S2. BCG reduced cytokine production after CS challenge but not after LPS.
  • Fig. S3. Representative flow cytometry plots of BCG-induced increase in total neutrophils and proportional increase in mature neutrophils.
  • Fig. S4. Graphical depiction of the adoptive transfer methods used.
  • Fig. S5. Adoptive transfers demonstrated the importance of BCG-induced neutrophils in combating sepsis.
  • Fig. S6. BCG-induced neutrophil expansion associated with the ability to confer protection from sepsis.
  • Fig. S7. BCG vaccination did not associate with greater lung damage, and BCG-induced neutrophil numbers correlated with greater protection.
  • Fig. S8. Various innate and adaptive immune system gene knockout mice still recognized BCG and were protected.
  • Fig. S9. BCG vaccination–induced EG-supporting cytokines, but only G-CSF was necessary and sufficient; steady-state granulopoiesis was unaffected by BCG.
  • Fig. S10. PCA visualization of 48 cytokines measured at different time points after vaccination.
  • Fig. S11. Representative flow cytometry plots of BCG-induced increase in GMPs.
  • Fig. S12. Validation of the BCG-induced signatures consistent with EG in human newborns.
  • Table S1. Cytokine involvement in EG, pro- or anti-inflammation, and as chemokines.
  • Table S2. Monitoring criteria for septic juvenile and adult mice.
  • References (55–118)

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Mouse data used in analysis.