Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Additional patient and cell line information.
  • Fig. S2. Additional WS4 stage 6 and human islet analysis.
  • Fig. S3. Differentiation progression and efficiency for WS4corr and WS4unedit lines.
  • Fig. S4. WFS1 expression during SC-β cell differentiation in WS4corr and WS4unedit lines.
  • Fig. S5. GSIS normalized to β cell population.
  • Fig. S6. Additional analysis of WS4corr and WS4unedit SC-β cell transplantations in diabetic mice.
  • Fig. S7. Additional analysis of WS4corr and WS4unedit SC-β cell scRNA-seq.
  • Fig. S8. Additional analysis of WS4corr and WS4unedit SC-β cell scRNA-seq for off-targets.
  • Fig. S9. Additional analysis of differences in SC-β cell β and islet markers for WS4corr clones and WS4unedit lines.
  • Fig. S10. Additional analysis of ER stress gene expression.
  • Fig. S11. Additional analysis of TEM and mitochondrial respiration.
  • Fig. S12. Treatment of SC-β cells with chemical stressors.
  • Fig. S13. Stress marker measurements of WS4corr-B and human islets.
  • Table S1. CRISPR sequences.
  • Table S2. Additional analysis of WS4corr and WS4unedit SC-β cell scRNA-seq and population up-regulated genes.
  • Table S3. Log fold change values between WS4corr and WS4unedit SC-β cells for markers in Figs. 5A and 6A.
  • Table S4. Differentiation protocol.
  • Table S5. Differentiation factors.
  • Table S6. Media and buffer formulations.
  • Table S7. Antibody list.
  • Table S8. Primers used for real-time PCR.
  • References (72, 73)

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Individual-level data for all figures.