Supplementary Materials

The PDF file includes:

  • Fig. S1. Viral loads according to duration of illness in infants with mild (outpatients) or severe (inpatients) RSV infection.
  • Fig. S2. Batch effect addressed using ComBat batch correction method.
  • Fig. S3. PCAs of transcriptome data from the inpatient and outpatient discovery cohorts to assess the impact of duration of symptoms on viral loads.
  • Fig. S4. Schematic figure of the interaction between RSV disease severity, viral loads, and age.
  • Fig. S5. Flow diagram of patient sample selection for transcriptome analyses.
  • Table S1. Demographic, clinical, and virologic data of 72 children included in the discovery age-matched cohort for transcriptome analyses.
  • Table S2. Demographic, clinical, and virologic data of 55 children included in the validation cohort for transcriptome analyses.
  • Table S3. Demographic and laboratory data of patients with RSV and healthy controls included in the blood cell immunophenotype analysis according to age (<6 months and 6 to 24 months of age).
  • Table S4. Demographic, clinical, and virologic data of 37 children included in the combined transcriptome and immunophenotype analysis.
  • Table S5. Specific and shared top over- and underexpressed transcripts included in the outpatient and inpatient biosignatures.
  • Table S6. Modular analyses of immune response related transcriptional modules.
  • Table S7. Top biological processes and pathways associated with the specific and shared RSV outpatient and inpatient biosignatures.
  • Table S8. Demographic, clinical, and virologic data of children <6 months of age included in the age group modular analyses.
  • Table S9. Demographic, clinical, and virologic data of children 6 to 24 months included in the modular analyses.
  • Table S10. Percentages of WBC subsets in infants with RSV infection and healthy controls according to age.
  • Table S11. Antibodies and fluorochromes used to stain WBC for immunophenotype analyses by multicolor flow cytometry.
  • Table S12. Cell surface markers used to define WBC populations by flow cytometry immunophenotyping.

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Other Supplementary Material for this manuscript includes the following: