Supplementary Materials

The PDF file includes:

  • Supplementary Methods
  • Fig. S1. Representative Western blot and quantification of LC3 in monocytes and of p62, pThr172-AMPKα, and pSer757-ULK1, in PBMCs from healthy donors and patients with cirrhosis.
  • Fig. S2. Lack of correlation between LAPosome number and ALT/AST in blood monocytes from patients with cirrhosis and representative profile of IL-8 expression in CD14+ monocytes from patients with cirrhosis by flow cytometry.
  • Fig. S3. LAP is increased in monocytes from mice with bile-duct ligation and Western diet and protects against hepatic inflammation and fibrosis.
  • Fig. S4. IgG-mediated activation of FcγRIIA underlies LAP-mediated anti-inflammatory signaling in monocytes from patients with cirrhosis: quantitative analysis and representative profiles.
  • Fig. S5. SHP-1 is recruited by FcγRIIA and transmits an anti-inflammatory signal: quantitative analysis.
  • Fig. S6. FcγRIIA-mediated activation of LAP as a potential therapeutic target: representative images and immunophenotyping.
  • Table S1. Characteristics of healthy donors and patients with cirrhosis used for studies in blood samples.
  • Table S2. Characteristics of patients with fibrosis used for studies in blood samples.
  • Table S3. Factors associated with LAPosome number.
  • Table S4. Characteristics of patients with ACLF.
  • Table S5. Primer sequences.
  • References (4145)

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Other Supplementary Material for this manuscript includes the following: