Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Characteristics of BMDMs.
  • Fig. S2. Effects of niacin in mouse macrophages and human monocytes: Cytokines, chemokines, and arginase activity.
  • Fig. S3. Niacin does not affect the viability or differentiation of monocytes.
  • Fig. S4. Reduction of BTIC growth by the conditioned medium of niacin-stimulated monocytes.
  • Fig. S5. Effects of Niacin-MonoCM or IFN-α14 on BTIC growth, cell cycle progression, apoptosis, and stemness.
  • Fig. S6. Effects of Niacin-MonoCM, niacin, and IFN-α14 on BTICs.
  • Fig. S7. Effect of mouse IFN-α14 on BT53M cells.
  • Fig. S8. IFN-α14 gene (IFNA14) expression and association with survival in GBM.
  • Fig. S9. TCGA analysis of other soluble factors.
  • Fig. S10. Clodronate liposome treatment depletes monocytes/macrophages as visualized by spinning-disc confocal microscopy of the liver.
  • Fig. S11. Peripheral and CNS infiltrating T lymphocytes in mice treated with niacin.
  • Fig. S12. Confirmation of mouse Ifnar1 gene KO by DNA sequencing.
  • Fig. S13. Effects of Niacin-MonoCM and temozolomide on BT048 growth in culture.
  • Fig. S14. Analysis of GBM patient–derived monocytes upon niacin stimulation.
  • References (61, 62)

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Differential gene expression in human monocytes upon niacin treatment (raw data).
  • Data file S2 (Microsoft Excel format). Human Interferon Pathway TaqMan Array of human BTIC lines upon treatment with IFN-α14.
  • Data file S3 (Microsoft Excel format). Raw data from experiments reported in composite figures.