Supplementary Materials

The PDF file includes:

  • Materials and methods
  • Fig. S1. Validation of rat anti-human/mouse PBX1 monoclonal antibody.
  • Fig. S2. The transcription factor PBX1 is more conserved than other related NK cell transcription factors.
  • Fig. S3. Expression of the GPFs, PTN and OGN, is up-regulated after overexpression of PBX1 in dNK-like cells.
  • Fig. S4. PBX1G21S retains DNA binding activity.
  • Fig. S5. dNK cells are the dominant subset expressing growth factors in early pregnancy.
  • Fig. S6. PBX1 is highly expressed in uterine NK cells from pregnant Pbx1HA-tag knock-in mice.
  • Fig. S7. Knockout of Pbx1 alters the gene expression profile in uterine NK cells, and the bone development of embryos is limited in pregnant Pbx1f/f;Ncr1Cre mice.
  • Fig. S8. Fetal growth is normal in pregnant EomesNK-KO mice.
  • Fig. S9. Working model of PBX1+ NK cells promoting early fetal development.
  • Table S1. Clinical data for patients with RSA and healthy controls.
  • Table S2. Commercial kits for cell preparation used in this study.
  • Table S3. Recombinant proteins used in this study.
  • Table S4. Antibodies used in this study.
  • Table S5. Human-specific primer sequences.
  • Table S6. Construction of siRNAs of the target gene.
  • Table S7. Primer sequences for the ChIP assay.
  • Table S8. Construction of oligonucleotides of Pbx1HA tag knock-in mice.

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Other Supplementary Material for this manuscript includes the following: