Supplementary Materials

The PDF file includes:

  • Fig. S1. Characterization of additional tracers and tracer delivery routes.
  • Fig. S2. Brief retinal exposure to trace amounts of hAβ using our intravitreal infusion paradigm does not induce gliosis, microglial activation, apoptosis or BRB disruption.
  • Fig. S3. Detailed analysis of ocular glymphatic clearance route.
  • Fig. S4. Validation of tracer delivery model in another species and with different infusion parameters.
  • Fig. S5. Light stimulation accelerates hAβ tracer movement along the optic nerve.
  • Fig. S6. Influence of the translaminar pressure difference on stimulated and unstimulated tracer movement.
  • Fig. S7. Tracer efflux pattern in murine glaucoma model indicates leaky lamina that may allow bypass of intra-axonal transport via extracellular route.
  • Fig. S8. Murine glaucoma models reveal defects in the lamina barrier allowing escape of large intraocular tracers.
  • Fig. S9. Schematic model of the ocular glymphatic clearance system and its dysfunction in a murine model of glaucoma.
  • Table S1. Retinal cytokine assay comparing intravitreal hAβ injection to vehicle.
  • Table S2. Comparison of the controls for the DBA/2J strain.
  • Table S3. Tracers used and their routes of administration.
  • Legend for movie S1

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). Animation of the proposed mechanism for ocular glymphatic clearance in the healthy optic nerve and glaucoma.
  • Data file S1. Tabular summary of all the data (provided as separate excel file).