Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Identification of TNBC breast cancers in TCGA and METABRIC datasets.
  • Fig. S2. Distribution of MYCN expression across TNBC.
  • Fig. S3. IHC detection of MYCN in CDX and PDX tissue.
  • Fig. S4. MYCN expression in primary TNBC and patient-matched metastases.
  • Fig. S5. MYCN and MYC intratumoral heterogeneity in primary and recurrent TNBC.
  • Fig. S6. MYCN and MYC expression in TNBC cell populations and CAL-51 clonally derived cell lines.
  • Fig. S7. BETi sensitivity of CAL-51 MYCNHigh cell lines.
  • Fig. S8. Changes in MYC target gene expression in CAL-51 MYCNHigh cell lines after BETi treatment.
  • Fig. S9. Differential gene expression analyses between MYCNRatioHigh and MYCRatioHigh TNBC.
  • Fig. S10. Effect of BETi and MEKi combination treatments on weight of treated mice.
  • Fig. S11. Evaluation of apoptosis and proliferation after BETi and MEKi treatment in TNBC PDX models.
  • Fig. S12. Evaluation of MYC family isoform expression after BETi and MEKi combination treatment in vivo.
  • Table S1. MYCN and MYC expression in breast cancer PDX models.
  • Table S2. Characteristics of patients with treatment-naïve and NAC-treated primary TNBC.
  • References (6679)

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Tabular data points for experiments with a sample size of n < 20.
  • Data file S2 (Microsoft Excel format). IHC results for MYCN and MYC in primary, treatment-naïve; primary, NAC-treated; and recurrent TNBC cases.
  • Data file S3 (Microsoft Excel format). Primary drug screen results using CAL-51 MYCNLow and MYCNHigh cell lines.
  • Data file S4 (Microsoft Excel format). Secondary drug screen results using CAL-51 MYCNLow and MYCNHigh cell lines.
  • Data file S5 (Microsoft Excel format). Tabular data points for MYC family isoform TSA-IF in CAL-51 after single-agent BETi treatment.
  • Data file S6 (Microsoft Excel format). Tabular data points for MYC family isoform TSA-IF in MDA-MB-468 after single-agent BETi treatment.
  • Data file S7 (Microsoft Excel format). Tabular data points for MYC family isoform TSA-IF in TNBC cell lines and PDX tissue after BETi and MEKi single-agent and combination treatment.