Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Gating strategy for ex vivo DC study from tumor and PBMC from patients with lung cancer and PBMC from healthy donors.
  • Fig. S2. PD-1, PD-L1, and B7.1 surface expression on peripheral DC subsets from healthy donors.
  • Fig. S3. B7.1 and PD-L1 are coexpressed on Clec9a+ DC from patients with RCC.
  • Fig. S4. Expression of PD-L1 and B7.1 by mono-DCs.
  • Fig. S5. PD-1 preferably colocalizes with CD28 in cis on T cells than with PD-L1 in trans within the immune synapses.
  • Fig. S6. B7.1 colocalizes preferably with PD-L1 in cis on DCs than with CD28 in trans within the synapses.
  • Fig. S7. Schematic diagram of anti–PD-L1 Ab clone 6E11.
  • Fig. S8. Quantification of PD-L1 and B7.1 on HEK cells.
  • Fig. S9. Anti–PD-L1–treated DCs enhances B7.1/CD28 colocalization within the DC:T cell synapses.
  • Fig. S10. Blocking PD-L1 on mouse DCs enhances their T cell priming capacity.
  • Fig. S11. PD-L1 blocking on DC enhances their T cell priming capacity.
  • Fig. S12. DC signature is associated with the improved survival benefit to atezolizumab in patients with NSCLC in a PD-L1–dependent manner.
  • Fig. S13. PD-L1 gene expression is not predictive for clinical benefit to atezolizumab.
  • Fig. S14. PD-L1 gene signature is not correlated with DC signature.
  • Fig. S15. Schematic drawing illustrating the modes of action of anti–PD-L1 mAb on DCs.
  • Table S1. Summary of surface expressions (specific antibody binding capacity) of B7.1, PD-1, and PD-L1 on tumor and peripheral DC subsets from patients with lung cancer.
  • Table S2. Relative contribution of PD-L1/B7.1 in cis interaction and PD-1/PD-L1 in trans interaction on CD28 signaling.
  • Table S3. Clinical response to atezolizumab in patients with RCC based on their DC gene signature.
  • Table S4. Clinical response to atezolizumab in patients with NSCLC based on their DC gene signature.
  • Table S5. Clinical response to atezolizumab in PD-L1+ (TC2/3 or IC2/3) patients with NSCLC based on their DC gene signature.
  • Legends for movies S1 and S2
  • Legend for data file S1

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). Three-dimensional movie construction of DC:T synapse of the confocal images from Fig. 3C.
  • Movie S2 (.mov format). Three-dimensional movie construction of DC:T synapse of the confocal images from Fig. 3C.
  • Data file S1 (Microsoft Excel format). Primary data.