Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Antigen expression and CLTX-Cy5.5 binding on GBMs.
  • Fig. S2. Activation of CLTX-CAR T cells after GBM stimulation.
  • Fig. S3. CLTX-EQ-28ζ and CLTX-CD8h-28ζ CARs initiate stronger T cell activity than other CLTX-CAR constructs.
  • Fig. S4. CLTX-CAR T cell targeting of GBM xenografts.
  • Fig. S5. CLTX-CAR T cells do not elicit off-tumor targeting in mouse models.
  • Fig. S6. MMP2 is necessary for CLTX-CAR T cell activation.
  • Legends for movies S1 and S2

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). Killing of PBT003-4-TS–derived GBMs during a 72-hour coculture with the mixture of IL13Rα2-, HER2-, and EGFRvIII-targeted CAR T cells.
  • Movie S2 (.avi format). Killing of PBT003-4-TS–derived GBMs during a 72-hour coculture with CLTX-EQ-28ζ CAR T cells.
  • Data file S1 (Microsoft Excel format). Primary data.
  • Data file S2 (Microsoft Excel format). Amino acid sequence of spacers.