Supplementary Materials

The PDF file includes:

  • Fig. S1. VitC quantification in plasma.
  • Fig. S2. High doses of VitC are required for maximal antiproliferative effects in murine breast tumors.
  • Fig. S3. Administration of antioxidant NAC does not impair VitC anticancer effect.
  • Fig. S4. Flow cytometry for IFN-γ in spleen-derived T lymphocytes.
  • Fig. S5. Isolation of T cells for adoptive T cell transfer.
  • Fig. S6. Depletion of CD4 T cells for adoptive cell transfer experiments.
  • Fig. S7. Tumor growth of individual mice treated as shown in Fig. 3 (A and B).
  • Fig. S8. Tumor growth of individual mice treated as shown in Fig. 3 (C to E).
  • Fig. S9. Modulation of tumor immune infiltration induced by VitC.
  • Fig. S10. Flow cytometry on CD44 and CD69 T cell markers on CD4 T lymphocytes.
  • Fig. S11. Representative CD44- and CD69-positive events on CD8 T lymphocytes by flow cytometry.
  • Fig. S12. VitC effect on growth of MMR-deficient tumors in NOD-SCID mice.
  • Fig. S13. Tumor growth of individual mice bearing MLH1-KO tumors and treated with ICT and VitC as shown in Fig. 5D.
  • Fig. S14. Tumor volume variations since treatment start of TS/A MLH1-KO and CT26 MLH1-KO tumors treated with ICT and VitC.

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Tumor measurements of experiments in Fig. 1.
  • Data file S2 (Microsoft Excel format). Tumor measurements of experiments in Fig. 2.
  • Data file S3 (Microsoft Excel format). Tumor measurements of experiments in Fig. 3.
  • Data file S4 (Microsoft Excel format). Immuno-phenotiping of CD4 and CD8 T cells.
  • Data file S5 (Microsoft Excel format). Tumor measurements of experiments in Fig. 5.
  • Data file S6 (Microsoft Excel format). Plasma VitC analysis.
  • Data file S7 (Microsoft Excel format). Tumor measurements of experiments in figs. S2 and S3.
  • Data file S8 (Microsoft Excel format). Tumor measurements of experiments in fig. S6.
  • Data file S9 (Microsoft Excel format). Tumor measurements of experiments in fig. S8.
  • Data file S10 (Microsoft Excel format). Tumor measurements of experiments in fig. S12.