Supplementary Materials

The PDF file includes:

  • Fig. S1. Clinical lymphocyte laboratory results from patients with AD.
  • Fig. S2. Signal intensities and gating for phenotypic analysis of human peripheral blood NK cells by CyTOF.
  • Fig. S3. Density plots of viSNE analysis of CyTOF data for Lin CD56+ cells by subject.
  • Fig. S4. Detailed signal intensities of CyTOF viSNE populations.
  • Fig. S5. RNA-seq of human CD56dim NK cells.
  • Fig. S6. Gating strategies for in vitro assays on CD56dim NK cells.
  • Fig. S7. AD NK cells demonstrate evidence of AICD.
  • Fig. S8. NK cell enrichment in AD skin of mice and humans.
  • Fig. S9. Gating strategy for murine skin immune cell populations.
  • Fig. S10. NK cell depletion results in enhanced ILC2 responses.
  • Fig. S11. IL-15 SA induces a dose-dependent NK cell expansion and ameliorates AD-like disease at later time points.
  • Fig. S12. Ncr1-DTA mice lack NK cells but have intact CD8 T cells.
  • Table S1. Demographics for subjects analyzed in Fig. 2, NK cell CyTOF.
  • Table S2. Demographics for subjects analyzed in Fig. 3, NK cell CyTOF before/after dupilumab treatment.
  • Table S3. Demographics for subjects analyzed in Fig. 3, plasma cytokines.
  • Table S4. Demographics for subjects analyzed in Fig. 4, NK cell RNA-seq.
  • Legends for tables S5 and S6
  • Table S7. Demographics for subjects analyzed in Fig. 4, cleaved caspase-3 staining.
  • Table S8. Demographics for subjects analyzed in Fig. 4, CD16 ligation.
  • Table S9. Demographics for subjects analyzed in Fig. 5, skin RNA-seq.
  • Legends for tables S10 and S11
  • Table S12. Human NK cell CyTOF antibodies.
  • Table S13. Flow cytometry antibodies.
  • Legend for data file S1

[Download PDF]

Other Supplementary Material for this manuscript includes the following:

  • Table S5 (.csv format). Differential gene expression table output from DESeq2 analysis of CD56dim NK cell RNA-seq.
  • Table S6 (.csv format). Differentially enriched GO terms from GAGE analysis of CD56dim NK cell RNA-seq.
  • Table S10 (.csv format). Differentially enriched GO terms from GAGE analysis of lesional versus nonlesional human skin RNA-seq.
  • Table S11 (.csv format). Differential gene expression table output from DESeq2 analysis of lesional versus nonlesional human skin RNA-seq.
  • Data file S1 (Microsoft Excel format). Primary data.