Supplementary Materials
The PDF file includes:
- Materials and Methods
- Fig. S1. Identification and characterization of lncRNA SNHG12 in mouse and human cells.
- Fig. S2. SNHG12 does not affect lipid metabolism or inflammation.
- Fig. S3. Identification of DNA-PK as an SNHG12 interactor.
- Fig. S4. SNHG12 silencing impairs DDR in ECs and macrophages.
- Fig. S5. Phenotypic effects of SNHG12 on senescence, EC permeability, and efferocytosis.
- Fig. S6. SNHG12 has no regulatory role in apoptosis.
- Fig. S7. Accumulating DNA damage and its effect on mitochondrial stress.
- Fig. S8. Proposed mechanism of SNHG12 regulation of atherosclerosis through a DNA-PK–mediated DDR in the vascular endothelium.
- Table S1. Primer list.
- References (59–63)
Other Supplementary Material for this manuscript includes the following:
- Data file S1 (Microsoft Excel format). Sequences for cloning.
- Data file S2 (Microsoft Excel format). Raw data from figures.
- Movie S1 (.mov format). Transcytosis assay of control gapmeR (25 nM)–transfected HCAECs.
- Movie S2 (.mov format). Transcytosis assay of SNHG12 gapmeR (25 nM)–transfected HCAECs.
- Movie S3 (.mov format). Transcytosis assay of control lentivirus–transduced HCAECs.
- Movie S4 (.mov format). Transcytosis assay of SNHG12 lentivirus–transduced HCAECs.
- Movie S5 (.mov format). Transcytosis assay of control lentivirus–transduced HCAECs in the presence of ROS.
- Movie S6 (.mov format). Transcytosis assay of SNHG12 lentivirus–transduced HCAECs in the presence of ROS.
