Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Identification and characterization of lncRNA SNHG12 in mouse and human cells.
  • Fig. S2. SNHG12 does not affect lipid metabolism or inflammation.
  • Fig. S3. Identification of DNA-PK as an SNHG12 interactor.
  • Fig. S4. SNHG12 silencing impairs DDR in ECs and macrophages.
  • Fig. S5. Phenotypic effects of SNHG12 on senescence, EC permeability, and efferocytosis.
  • Fig. S6. SNHG12 has no regulatory role in apoptosis.
  • Fig. S7. Accumulating DNA damage and its effect on mitochondrial stress.
  • Fig. S8. Proposed mechanism of SNHG12 regulation of atherosclerosis through a DNA-PK–mediated DDR in the vascular endothelium.
  • Table S1. Primer list.
  • References (5963)

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Sequences for cloning.
  • Data file S2 (Microsoft Excel format). Raw data from figures.
  • Movie S1 (.mov format). Transcytosis assay of control gapmeR (25 nM)–transfected HCAECs.
  • Movie S2 (.mov format). Transcytosis assay of SNHG12 gapmeR (25 nM)–transfected HCAECs.
  • Movie S3 (.mov format). Transcytosis assay of control lentivirus–transduced HCAECs.
  • Movie S4 (.mov format). Transcytosis assay of SNHG12 lentivirus–transduced HCAECs.
  • Movie S5 (.mov format). Transcytosis assay of control lentivirus–transduced HCAECs in the presence of ROS.
  • Movie S6 (.mov format). Transcytosis assay of SNHG12 lentivirus–transduced HCAECs in the presence of ROS.