Supplementary Materials

The PDF file includes:

• Fig. S1. Characterization of purified and probiotically produced PD-L1nb.
• Fig. S2. Maps of plasmids.
• Fig. S3. Individual growth trajectories of tumors treated with lysis circuit variants.
• Fig. S4. Individual growth trajectories of tumors treated with different probiotic therapy regimens.
• Fig. S5. Characterization of intratumorally and systemically delivered probiotic therapy.
• Fig. S6. Individual tumor trajectories of multiple- and single-dose treatment regimens in a syngeneic colorectal cancer model.
• Fig. S7. Tumor trajectories of probiotic and antibody therapeutics in a syngeneic colorectal cancer model.
• Fig. S8. Histological images and dirty necrosis scoring in murine colorectal cancer tumors.
• Fig. S9. Growth trajectories of colorectal tumors treated with SLIC-3.

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Other Supplementary Material for this manuscript includes the following:

• Data file S1 (Microsoft Excel format). Raw data for composite graphs.