Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Overview of blood sampling at 18 months of age.
  • Fig. S2. Gating strategy for enumeration of immune cells by flow cytometry.
  • Fig. S3. Correlations between relative cell frequencies and absolute cell counts.
  • Fig. S4. Multivariate analysis of cytokine profiles from innate ligand-stimulated blood collected at 18 months of age.
  • Fig. S5. Hierarchical clustering of the functional response profile to innate ligands in blood immune cells from 18-month-old infants.
  • Fig. S6. Distinct antimicrobial innate response profiles in infants associate to overall risk of childhood asthma.
  • Fig. S7. Reduced risk of transient childhood asthma in infants within the NLRP3-C2 at 18 months of age.
  • Fig. S8. Early immune phenotypes in infants that develop persistent childhood asthma.
  • Fig. S9. Hierarchical clustering of the functional response profile in blood αβ T cells from 18-month-old infants.
  • Table S1. Demographic data of study cohort.
  • Table S2. Relative frequencies of immune cell subsets in whole blood at 18 months of age.
  • Table S3. Whole-blood concentration of immune cell subsets in 18-month-old infants.
  • Table S4. Concentrations of cytokines and chemokines after 24 hours incubation with media only (unstimulated, i.e., spontaneous release) or ligands targeting the indicated receptors.
  • Table S5. Genetic and environmental determinants of TLR3 low responders.
  • Table S6. Genetic and environmental determinants of TLR4 low responders.
  • Table S7. Genetic and environmental determinants of TLR7/8 low responders.
  • Table S8. Genetic and environmental determinants of NOD2 low responders.
  • Table S9. Genetic and environmental determinants of NLRP3 low responders.
  • Table S10. Genetic and environmental determinants of αβTCR low responders.
  • Table S11. Genetic and environmental determinants of transient and persistent asthma.
  • Table S12. Genetic and environmental determinants of at-risk TLR7/8-C7 immune cluster for transient asthma.
  • Legend for data file S1

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Intra-individual matrix comparing the response type to the antiviral and antibacterial ligands and the T cell response type.