Supplementary Materials

The PDF file includes:

  • Supplementary Materials and Methods
  • Fig. S1. mGluR1 expression in the postmortem ET cerebellum.
  • Fig. S2. The amino acid alignment of the protein products of WT and hotfoot17J GluRδ2 cDNA.
  • Fig. S3. Quantification of cellular and subcellular expression of GluRδ2 in WT and Grid2dupE3 cerebellum.
  • Fig. S4. Suppression of mouse tremor by ET medications.
  • Fig. S5. Regression of CF synapses after rescue of GluRδ2 expression.
  • Fig. S6. Tremor in Grid2dupE3 mice receiving intracerebellar injection of control SINV.
  • Fig. S7. Tremor in hotfoot4J mice.
  • Fig. S8. Cryoinjury of mouse cerebellum by dry ice.
  • Fig. S9. Frequency profiles of tremor in Grid2dupE3 mice by IO silencing.
  • Fig. S10. Spike-phase coupling between IO spikes and tremor phases.
  • Fig. S11. Spike-phase coupling between IO spikes and cerebellar LFPs.
  • Fig. S12. Suppression of cerebellar oscillations and tremor by IO silencing.
  • Fig. S13. Optogenetic stimulation of PC outputs at 10 Hz.
  • Fig. S14. Optogenetic stimulation of PC outputs by nonactivating green light.
  • Fig. S15. Signals and characteristics of cerebellar EEG leads and nearby muscle leads.
  • Fig. S16. Signal comparison between cerebellar EEG leads and nearby muscle leads in patients with ET.
  • Fig. S17. Occipital alpha rhythm and cerebellar oscillations in a patient with ET.
  • Fig. S18. CF synaptic pruning deficits, cerebellar oscillations, and tremor.
  • Table S1. Clinical and pathological features of postmortem cerebellum in patients with ET and control subjects.
  • Table S2. Demographic data of patients with ET and control subjects for the EEG study.
  • Table S3. Demographic data of patients with ET and control subjects for cerebellar oscillation index.
  • Legends for movies S1 to S5
  • Legend for data file S1
  • References (6467)

[Download PDF]

Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). Tremor characteristics in WT versus Grid2dupE3 mice.
  • Movie S2 (.mp4 format). Tremor modulation by dry ice–mediated cerebellar lesioning.
  • Movie S3 (.mp4 format). Tremor modulation by optogenetic inhibition of cerebellar outputs.
  • Movie S4 (.mp4 format). Tremor modulation by synaptic inhibition of CFs.
  • Movie S5 (.mp4 format). Tremor induction by PC stimulation in a WT mouse.
  • Data file S1. Raw data (provided as separate excel file).