Supplementary Materials
The PDF file includes:
- Supplementary Materials and Methods
- Fig. S1. mGluR1 expression in the postmortem ET cerebellum.
- Fig. S2. The amino acid alignment of the protein products of WT and hotfoot17J GluRδ2 cDNA.
- Fig. S3. Quantification of cellular and subcellular expression of GluRδ2 in WT and Grid2dupE3 cerebellum.
- Fig. S4. Suppression of mouse tremor by ET medications.
- Fig. S5. Regression of CF synapses after rescue of GluRδ2 expression.
- Fig. S6. Tremor in Grid2dupE3 mice receiving intracerebellar injection of control SINV.
- Fig. S7. Tremor in hotfoot4J mice.
- Fig. S8. Cryoinjury of mouse cerebellum by dry ice.
- Fig. S9. Frequency profiles of tremor in Grid2dupE3 mice by IO silencing.
- Fig. S10. Spike-phase coupling between IO spikes and tremor phases.
- Fig. S11. Spike-phase coupling between IO spikes and cerebellar LFPs.
- Fig. S12. Suppression of cerebellar oscillations and tremor by IO silencing.
- Fig. S13. Optogenetic stimulation of PC outputs at 10 Hz.
- Fig. S14. Optogenetic stimulation of PC outputs by nonactivating green light.
- Fig. S15. Signals and characteristics of cerebellar EEG leads and nearby muscle leads.
- Fig. S16. Signal comparison between cerebellar EEG leads and nearby muscle leads in patients with ET.
- Fig. S17. Occipital alpha rhythm and cerebellar oscillations in a patient with ET.
- Fig. S18. CF synaptic pruning deficits, cerebellar oscillations, and tremor.
- Table S1. Clinical and pathological features of postmortem cerebellum in patients with ET and control subjects.
- Table S2. Demographic data of patients with ET and control subjects for the EEG study.
- Table S3. Demographic data of patients with ET and control subjects for cerebellar oscillation index.
- Legends for movies S1 to S5
- Legend for data file S1
- References (64–67)
Other Supplementary Material for this manuscript includes the following:
- Movie S1 (.mp4 format). Tremor characteristics in WT versus Grid2dupE3 mice.
- Movie S2 (.mp4 format). Tremor modulation by dry ice–mediated cerebellar lesioning.
- Movie S3 (.mp4 format). Tremor modulation by optogenetic inhibition of cerebellar outputs.
- Movie S4 (.mp4 format). Tremor modulation by synaptic inhibition of CFs.
- Movie S5 (.mp4 format). Tremor induction by PC stimulation in a WT mouse.
- Data file S1. Raw data (provided as separate excel file).