Supplementary Materials

The PDF file includes:

• Supplementary Materials and Methods
• Fig. S1. mGluR1 expression in the postmortem ET cerebellum.
• Fig. S2. The amino acid alignment of the protein products of WT and hotfoot17J GluRδ2 cDNA.
• Fig. S3. Quantification of cellular and subcellular expression of GluRδ2 in WT and Grid2^dupE3 cerebellum.
• Fig. S4. Suppression of mouse tremor by ET medications.
• Fig. S5. Regression of CF synapses after rescue of GluRδ2 expression.
• Fig. S6. Tremor in Grid2^dupE3 mice receiving intracerebellar injection of control SINV.
• Fig. S7. Tremor in hotfoot4J mice.
• Fig. S8. Cryoinjury of mouse cerebellum by dry ice.
• Fig. S9. Frequency profiles of tremor in Grid2^dupE3 mice by IO silencing.
• Fig. S10. Spike-phase coupling between IO spikes and tremor phases.
• Fig. S11. Spike-phase coupling between IO spikes and cerebellar LFPs.
• Fig. S12. Suppression of cerebellar oscillations and tremor by IO silencing.
• Fig. S13. Optogenetic stimulation of PC outputs at 10 Hz.
• Fig. S14. Optogenetic stimulation of PC outputs by nonactivating green light.
• Fig. S15. Signals and characteristics of cerebellar EEG leads and nearby muscle leads.
• Fig. S16. Signal comparison between cerebellar EEG leads and nearby muscle leads in patients with ET.
• Fig. S17. Occipital alpha rhythm and cerebellar oscillations in a patient with ET.
• Fig. S18. CF synaptic pruning deficits, cerebellar oscillations, and tremor.
• Table S1. Clinical and pathological features of postmortem cerebellum in patients with ET and control subjects.
• Table S2. Demographic data of patients with ET and control subjects for the EEG study.
• Table S3. Demographic data of patients with ET and control subjects for cerebellar oscillation index.
• Legends for movies S1 to S5
• Legend for data file S1
• References (64–67)

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Other Supplementary Material for this manuscript includes the following:

• Movie S1 (.mp4 format). Tremor characteristics in WT versus Grid2^dupE3 mice.
• Movie S2 (.mp4 format). Tremor modulation by dry ice–mediated cerebellar lesioning.
• Movie S3 (.mp4 format). Tremor modulation by optogenetic inhibition of cerebellar outputs.
• Movie S4 (.mp4 format). Tremor modulation by synaptic inhibition of CFs.
• Movie S5 (.mp4 format). Tremor induction by PC stimulation in a WT mouse.
• Data file S1. Raw data (provided as separate excel file).