Supplementary Materials

The PDF file includes:

  • Fig. S1. Murine cell lines show different sensitivities to oncolytic vaccinia virus in vitro.
  • Fig. S2. Combined intratumoral administration of hIL-7-VV and mIL-12-VV induces dose-dependent secretion of IL-7, IL-12, and IFN-γ in tumors.
  • Fig. S3. Combination of hIL-7-VV and mIL-12-VV does not cause weight loss in the LLC model.
  • Fig. S4. Combined intratumoral administration of hIL-7-VV and mIL-12-VV shows dose-dependent antitumor efficacy.
  • Fig. S5. hIL-7-VV shows superior antitumor activity to mGM-CSF-VV in combination with mIL-12-VV in the LLC model.
  • Fig. S6. Intratumoral administration of hIL-7/mIL-12-VV induces complete tumor regression in the CT26.WT model.
  • Fig. S7. CD4+ T cell depletion does not affect antitumor activity of hIL-7/mIL-12-VV in the CT26.WT model.
  • Fig. S8. Treatment with hIL-7/mIL-12-VV up-regulates gene expression related to broad immune pathways.
  • Fig. S9. Intratumoral administration of hIL-7/mIL-12-VV increases intratumoral infiltration of MDSCs.
  • Fig. S10. Intratumoral administration of hIL-7/mIL-12-VV changes immune status of noninjected distant tumors in the LLC model.
  • Fig. S11. Intratumoral administration of hIL-7/mIL-12-VV in combination with systemic checkpoint inhibitors does not change body weight in mice.
  • Fig. S12. Intratumoral administration of hIL-7/mIL-12-VV induces the establishment of antitumor memory in the LLC syngeneic murine model.
  • Fig. S13. Intratumoral administration of hIL-7/hIL-12-VV induces secretion of IL-7 and IL-12.
  • Legends for data files S1 to S4

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Up-regulation of genes related to T cell function pathway (hIL-7/mIL-12-VV versus Cont-VV).
  • Data file S2 (Microsoft Excel format). Up-regulation of genes related to antigen processing pathway (hIL-7/mIL-12-VV versus Cont-VV).
  • Data file S3 (Microsoft Excel format). Cell lines used in this study.
  • Data file S4 (Microsoft Excel format). Original data.