Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Differentiation bias of transplanted human NPCs to astrocytes.
  • Fig. S2. Increase in the amount proastrocytic differentiation factors after SCI.
  • Fig. S3. Functional interaction network for differentially expressed genes in hiPSC-NPCs after treatment with GDNF.
  • Fig. S4. Western blot confirmation of knocking out of DLK1 in hiPSC-NPCs.
  • Fig. S5. Expression and secretion of GDNF into tissue from transplanted cells.
  • Fig. S6. Reduced death in GDNF-expressing hiPSC-NPCs after exposure to injured spinal cord homogenate.
  • Fig. S7. Quantification of DLK1, Hes1, and Ki67 staining.
  • Fig. S8. Immunoelectron microscopy to analyze the myelination by transplanted cells.
  • Fig. S9. Staining for neuron-specific subtypes and human cell–specific marker.
  • Fig. S10. The effect of GDNF secretion from hiPSC-NPCs on host tissue preservation.
  • Fig. S11. Evaluation of thermal and mechanical allodynia.
  • Table S1. List of antibodies.
  • Table S2. List of Applied Biosystems TaqMan probes used for qPCR.
  • Table S3. List of growth factors and cytokines used for screening.
  • References (76–85)

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Other Supplementary Material for this manuscript includes the following: