Supplementary Materials
The PDF file includes:
- Materials and Methods
- Fig. S1. miR-218 is highly and specifically expressed in human and murine spinal motor neurons.
- Fig. S2. High content analysis of neuronal morphology after miR-218 perturbation.
- Fig. S3. miR-218 regulates intrinsic excitability.
- Fig. S4. qPCR validation of miR-218 target KD.
- Fig. S5. Evaluation of miR-218 upstream of the mRNA encoding for the potassium channel Kv4.2 (Kcnd2).
- Fig. S6. Kv10.1 (KCNH1) protein quantification by Western blot after miR-218 KD.
- Fig. S7. A summary diagram of key observations.Table S1. Identified hsa-miR-218-2 variants.
- Table S2. DsiRNA sequences used in the study.
- Table S3. Synthetic miR-218 sequences used for cloning into pMA-T vectors.
- Table S4. Primers used for quantitative real-time PCR.
- Legends for data files S1 to S4
- References (61–72)
Other Supplementary Material for this manuscript includes the following:
- Data file S1 (Microsoft Excel format). Individual-level data for miR-218 expression.
- Data file S2 (Microsoft Excel format). NanoString nCounter data for miRNAs measured in lumbar ventral horns.
- Data file S3 (Microsoft Excel format). Source data for Kv10.1 (KCNH1) Western blot studies.
- Data file S4 (Microsoft Excel format). Source data for Kv4.2 (KCND2) Western blot studies.
