Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. miR-218 is highly and specifically expressed in human and murine spinal motor neurons.
  • Fig. S2. High content analysis of neuronal morphology after miR-218 perturbation.
  • Fig. S3. miR-218 regulates intrinsic excitability.
  • Fig. S4. qPCR validation of miR-218 target KD.
  • Fig. S5. Evaluation of miR-218 upstream of the mRNA encoding for the potassium channel Kv4.2 (Kcnd2).
  • Fig. S6. Kv10.1 (KCNH1) protein quantification by Western blot after miR-218 KD.
  • Fig. S7. A summary diagram of key observations.Table S1. Identified hsa-miR-218-2 variants.
  • Table S2. DsiRNA sequences used in the study.
  • Table S3. Synthetic miR-218 sequences used for cloning into pMA-T vectors.
  • Table S4. Primers used for quantitative real-time PCR.
  • Legends for data files S1 to S4
  • References (6172)

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Individual-level data for miR-218 expression.
  • Data file S2 (Microsoft Excel format). NanoString nCounter data for miRNAs measured in lumbar ventral horns.
  • Data file S3 (Microsoft Excel format). Source data for Kv10.1 (KCNH1) Western blot studies.
  • Data file S4 (Microsoft Excel format). Source data for Kv4.2 (KCND2) Western blot studies.