Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. PDCD10 deficiency in brain endothelial cell signaling in mice and the gut microbiome in humans compared to KRIT1 and CCM2.
  • Fig. S2. Single-cell RNA sequencing of the intestinal epithelium shows broad expression of Krit1, Ccm2, and Pdcd10 across cell types.
  • Fig. S3. Analysis of the hindbrain upon deletion of Pdcd10 solely in IECs.
  • Fig. S4. Quantification of the colonic mucus layer.
  • Fig. S5. Gut epithelial loss of CCM2 or MAP3K3 and subsequent effects on the colonic mucus barrier and fecal LCN2.
  • Fig. S6. Measurement of Krit1, Ccm2, and Pdcd10 mRNA in the colonic epithelium.
  • Fig. S7. Analysis of goblet cell numbers, MUC2 expression, and colonic crypt morphology after intestine epithelial deletion of Krit1, Ccm2, or Pdcd10.
  • Fig. S8. E-cadherin (CDH1) expression and localization in colons lacking PDCD10, KRIT1, or MUC2.
  • Fig. S9. EpCAM expression and localization in colons lacking PDCD10, KRIT1, or MUC2.
  • Fig. S10. Intestinal junctions in PDCD10-deficient embryonic zebrafish.
  • Fig. S11. Heatmaps of 16S rRNA bacterial gene sequencing results for each animal by cage/litter.
  • Fig. S12. Unweighted principal component plots of 16S rRNA bacterial gene sequencing.
  • Fig. S13. Relative abundance boxplots of changed bacterial taxa as detected by 16S rRNA bacterial gene sequencing.
  • Fig. S14. Heatmaps of 16S rRNA bacterial gene sequencing results for the P80 emulsifier experiment by treatment group and cage.
  • Fig. S15. Dexamethasone effects on CCMs and colonic goblet cells.
  • Fig. S16. Model of the role of PDCD10 in the gut epithelium and its effect on CCM formation in the brain.
  • Table S1. Clinical characteristics of patients with CCM recruited to participate in gut microbiome study.
  • References (5569)

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