Supplementary Materials

The PDF file includes:

• Materials and Methods
• Fig. S1. PDCD10 deficiency in brain endothelial cell signaling in mice and the gut microbiome in humans compared to KRIT1 and CCM2.
• Fig. S2. Single-cell RNA sequencing of the intestinal epithelium shows broad expression of Krit1, Ccm2, and Pdcd10 across cell types.
• Fig. S3. Analysis of the hindbrain upon deletion of Pdcd10 solely in IECs.
• Fig. S4. Quantification of the colonic mucus layer.
• Fig. S5. Gut epithelial loss of CCM2 or MAP3K3 and subsequent effects on the colonic mucus barrier and fecal LCN2.
• Fig. S6. Measurement of Krit1, Ccm2, and Pdcd10 mRNA in the colonic epithelium.
• Fig. S7. Analysis of goblet cell numbers, MUC2 expression, and colonic crypt morphology after intestine epithelial deletion of Krit1, Ccm2, or Pdcd10.
• Fig. S8. E-cadherin (CDH1) expression and localization in colons lacking PDCD10, KRIT1, or MUC2.
• Fig. S9. EpCAM expression and localization in colons lacking PDCD10, KRIT1, or MUC2.
• Fig. S10. Intestinal junctions in PDCD10-deficient embryonic zebrafish.
• Fig. S11. Heatmaps of 16S rRNA bacterial gene sequencing results for each animal by cage/litter.
• Fig. S12. Unweighted principal component plots of 16S rRNA bacterial gene sequencing.
• Fig. S13. Relative abundance boxplots of changed bacterial taxa as detected by 16S rRNA bacterial gene sequencing.
• Fig. S14. Heatmaps of 16S rRNA bacterial gene sequencing results for the P80 emulsifier experiment by treatment group and cage.
• Fig. S15. Dexamethasone effects on CCMs and colonic goblet cells.
• Fig. S16. Model of the role of PDCD10 in the gut epithelium and its effect on CCM formation in the brain.
• Table S1. Clinical characteristics of patients with CCM recruited to participate in gut microbiome study.
• References (55–69)

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Other Supplementary Material for this manuscript includes the following:

• Data file S1 (Microsoft Excel format). Raw data.