Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Influence of old donor gut microbiota transplants on young germ-free recipients.
  • Fig. S2. Systemic effects of old donor gut microbiota transplants on young germ-free recipients.
  • Fig. S3. Analysis of neuronal markers in gut microbiota–transplanted recipients and their donors.
  • Fig. S4. Assessment of intestinal morphology in gut microbiota–transplanted recipients and their donors.
  • Fig. S5. Metabolic effects of old and young donor gut microbiota transplants on germ-free recipients.
  • Fig. S6. Comparative analysis of young gut microbiota–transplanted recipient mice and conventionally housed mice with a normal gut microbiota.
  • Fig. S7. Impact of young or old donor gut microbiota transplant on old germ-free recipients.
  • Fig. S8. Impact of sodium butyrate treatment on germ-free mice.
  • Fig. S9. Schematic representation of possible pathways involved in the increased hippocampal neurogenesis and prolongevity signatures observed in old MT recipient mice.
  • Table S1. Altered metabolites in the liver of old MT recipient mice measured using 1H NMR.
  • Table S2. Details of primers used for RT-PCR analysis of mouse tissues.

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Individual-level data for Fig. 1.
  • Data file S2 (Microsoft Excel format). Individual-level data for Fig. 2.
  • Data file S3 (Microsoft Excel format). Individual-level data for Fig. 3.
  • Data file S4 (Microsoft Excel format). Individual-level data for Fig. 4.
  • Data file S5 (Microsoft Excel format). Individual-level data for Fig. 5.