Supplementary Materials

The PDF file includes:

  • Fig. S1. Growth of Nluc C. parvum strains in epithelial cells and efficacy of AN3661 in adult IL12p40−/− and neonatal mice.
  • Fig. S2. Domain architecture and sequence conservation of C. hominis CPSF3.
  • Fig. S3. Structural model of ChCPSF3 with an mRNA substrate docked into the catalytic site.
  • Fig. S4. Hydrophobic belt around the residues coordinating the Zn atoms and stereo view of the electron density of metals.
  • Fig. S5. Stereo view of the catalytic site of ChCPSF3 with electron density of AN3661.
  • Fig. S6. Detailed view of the interactions established by AN3661 at the catalytic site of ChCPSF3.
  • Fig. S7. Structural differences between Cryptosporidium and human CPSF3.
  • Fig. S8. Conservation of the CPSF3 AN3661-binding site in apicomplexan and trypanosomatid parasites.
  • Fig. S9. Summary of mRNA processing machinery and inhibition of parasite CPSF3 by oxaboroles.
  • Table S1. Crystallographic data and refinement statistics.
  • Legend for movie S1

[Download PDF]

Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). Proposed inhibition mechanism of CPSF3 by the benzoxaborole AN3661.