Supplementary Materials

The PDF file includes:

• Materials and Methods
• Fig. S1. Expression of embryonic CaVβ1 isoform.
• Fig. S2. CaVβ1 isoform localization on adult skeletal muscle.
• Fig. S3. Histological characterization and myogenin signaling in CaVβ1E knockdown muscles.
• Fig. S4. Expression of Cacnb1-E and Gdf5 in C2C12.
• Fig. S5. Effects of CaVβ1E, CaVβ1D, and GDF5 overexpression on AAV-ShCaVβ1E–treated muscles.
• Fig. S6. GDF5 overexpression in aged mice muscle.
• Fig. S7. CaVβ1E overexpression in young mice muscle.
• Fig. S8. GDF5 overexpression in young mice muscle.
• Fig. S9. Schematic representation of CaVβ1E/GDF axis in skeletal muscle.
• Table S1. Expression data.
• Table S2. The 100 top up-regulated genes in innervated and denervated samples.
• Table S3. List of human samples.
• Table S4. Primer list.
• Legends for data files S1 to S3

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Other Supplementary Material for this manuscript includes the following:

• Data file S1 (Microsoft Excel format). List of 1022 differentially regulated alternative splicing events.
• Data file S2 (.pdf format). Uncut Western blotting raw data.
• Data file S3 (Microsoft Excel format). Individual participant data.