Supplementary Materials

This PDF file includes:

• Materials and Methods
• Fig. S1. AKOS02428449 activity against HEVA71.
• Fig. S2. Cytotoxicity profiling of bone marrow muscle (SJ) cells with prunin.
• Fig. S3. Classification of flavonoids.
• Table S1. Hit list of flavonoids generated from luciferase-screening assay, which are ranked according to their percentage inhibitions of IRES activity.
• Table S2. Clinical scoring system for the EV71 BALB/c mouse model.
• Table S3. Silent mutations found in the genomes of HEVA71 prunin-resistant mutants.
• Table S4. Structures and classification of the seven chosen flavonoids.
• Table S5. The primers (HEV1–16) used for DNA sequencing of the modified HEVA71 IRES bicistronic reporter construct.

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