Supplementary Materials

The PDF file includes:

  • Fig. S1. PK of NHC and EIDD-2801 in mice.
  • Fig. S2. Single-dose PK of EIDD-2801 in ferrets.
  • Fig. S3. Multidose PK of EIDD-2801 in ferrets.
  • Fig. S4. Ferret efficacy study timeline.
  • Fig. S5. Histopathology scores of Ca09-infected ferret lungs.
  • Fig. S6. Escalating-dose adaptation of IAV to NHC.
  • Fig. S7. Fixed-dose serial passaging of IAV in the presence of NHC.
  • Fig. S8. Genetic changes in IAV-WSN RNA during fixed-dose passaging.
  • Fig. S9. Immunofluorescence of influenza-infected 3D airway epithelium cultures.
  • Fig. S10. Therapeutic efficacy of NHC in the 3D airway epithelium culture.
  • Fig. S11. Cytotoxicity of NHC in the 3D airway epithelium culture.
  • Fig. S12. NHC effect on nuclear and mitochondrial gene expressions.
  • Fig. S13. Recapitulation of NHC PK profiles in 3D human airway epithelium culture.
  • Fig. S14. Immunohistochemistry of nasal turbinates extracted from vehicle- and EIDD-2801–treated animals.
  • Fig. S15. Immunohistochemistry of lungs extracted from vehicle- and EIDD-2801–treated animals.
  • Fig. S16. Immunofluorescence of 3D airway epithelium cultures.
  • Fig. S17. Immunofluorescence of 3D airway epithelium cultures after NHC exposure.
  • Table S1. PK parameters for NHC in cynomolgus macaques.
  • Table S2. Single-dose PK parameters for NHC in ferrets.
  • Table S3. Lung concentrations of NHC and NHC-TP.
  • Table S4. Multidose PK parameters for NHC in ferrets.
  • Table S5. Antibodies used in this study.
  • Table S6. Primers used in this study.
  • Legends for data files S1 to S5

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (.html format). Amino acid changes during baloxavir adaptation.
  • Data file S2 (Microsoft Excel format). Summary of amino acid changes during baloxavir adaptation.
  • Data file S3 (.html format). Amino acid changes during NHC adaptation.
  • Data file S4 (Microsoft Excel format). Summary of amino acid changes during NHC adaptation.
  • Data file S5 (Microsoft Excel format). Primary data.