Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Simoa calibration curve for four cytokines using the four-plex assay with standards prepared in calibrator diluent, plotted on a log-log scale.
  • Fig. S2. Plots of AEB against concentrations of cytokines for four different unique bead types in a four-plex Simoa assay.
  • Fig. S3. Precision profile of the four-plex cytokine assay.
  • Table S1. Coupling confirmation for Ag85B antigen.
  • Table S2. Inter-run precision of AEB values for four cytokines over three individual runs on different days with triplicate measurements per run.
  • Table S3. Interassay variation of four-plex cytokine assay.
  • Table S4. Interassay variation of Ag85B serological assay.
  • Table S5. Results for spike and recovery tests in real human serum samples using a fourfold dilution with sample diluent.
  • References (4656)

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Inflammation map.
  • Data file S2 (Microsoft Excel format). Luminex data.
  • Data file S3 (Microsoft Excel format). Four cytokine Simoa data of Broad plasma samples.
  • Data file S4 (Microsoft Excel format). Four cytokine Simoa data of FIND plasma and serum samples.
  • Data file S5 (Microsoft Excel format). Four cytokine and Ag85B IgG Simoa data of tested samples (combined Broad and FIND).