Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Assessment of PCA-mediated toxicity on HEK-293 cells.
  • Fig. S2. Optimization of the PCAON system in HEK-293 cells.
  • Fig. S3. PCA analog-mediated SEAP expression in pJY14/pJY29-transgenic HEK-293 cells.
  • Fig. S4. PCAON-dependent SEAP expression kinetics in HEK-293 cells.
  • Fig. S5. Design, construction, and optimization of the PcaR-mediated inhibition device (PcaRi) for gene inhibition.
  • Fig. S6. Design, construction, and optimization of the PcaR-mediated activation device (PcaRa) for gene activation.
  • Fig. S7. Schematics of a synthetic PcaR-mediated gene deletion device (PcaRdel).
  • Fig. S8. Controls with constitutively active CRISPR-dCas9 device-mediated genome repression and activation.
  • Fig. S9. PCA- and VA-controlled programmable biocomputers in mammalian cells.
  • Fig. S10. Flow cytometric histograms showing input-triggered single-cell d2EYFP expression of all programmed logic circuits.
  • Fig. S11. Validation of the VAON and the VAOFF system in mammalian cells.
  • Fig. S12. Validation of the PCAOFF system in mammalian cells.
  • Fig. S13. Construction and characterization of the stable cell lines.
  • Fig. S14. PCAON-2.0 switch-controlled treatment in type 1 diabetic mice by oral delivery of PCA.
  • Fig. S15. Hypoglycemic effect on type 1 and type 2 diabetic mice by oral administration of PCA or tea drinking.
  • Table S1. The CBC and blood biochemistry tests in type 1 diabetic monkeys.
  • Table S2. The CBC and blood biochemistry tests in type 2 diabetic monkeys.
  • Table S3. Plasmids designed and used in this study.
  • Table S4. The primers used for qPCR analysis.
  • Table S5. The primers used for PCR amplification.
  • Table S6. The expression vectors and mixtures for logic gates in mice.
  • Table S7. The expression vectors and mixtures for logic gates in mammalian cells.
  • References (5364)

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). DNA sequence information of plasmids used in this study.
  • Data file S2 (Microsoft Excel format). Individual subject-level data.