Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Data to support assessments of astrocyte reactivity in human and mouse HD shown in Fig. 1.
  • Fig. S2. Rpl22-HA expression in dorsolateral striatum.
  • Fig. S3. Overall numbers of differentially expressed astrocyte genes for Q175 and R6/2 mouse models versus their cognate controls.
  • Fig. S4. Astrocyte differential gene expression in Q175 versus WT and R6/2 versus NCAR at the three stages of study.
  • Fig. S5. Overall numbers of differentially expressed astrocyte genes in HD versus control with the application of different threshold criteria to analyze the data.
  • Fig. S6. Assessing astrocyte reactivity in HD mouse models with RNA-seq.
  • Fig. S7. Summary data showing how chosen pathways of interest in astrocyte biology change in mouse models of HD.
  • Fig. S8. Pathways altered within astrocytes in HD model mice.
  • Fig. S9. Assessments of early gene expression changes in HD.
  • Fig. S10. WGCNA to identify progressive alterations in astrocytes from HD mouse models.
  • Fig. S11. WGCNA modules with gene expression profiles that go in opposite directions in controls when compared to HD mice.
  • Fig. S12. Common DEGs between Q175 versus WT and R6/2 versus NCAR.
  • Fig. S13. Differential expression of the 62-core genes identified in the main manuscript in the allelic series proteomics datasets.
  • Fig. S14. Altered relative expression of the top 50 DEGs from 3-month R6/2 mice in ZFPDelta and ZFP groups.
  • Legends for data files S1 to S5

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). DEGs with FDR < 0.05 and FPKM > 10 for Q175 versus WT at 2, 6, and 12 months of age.
  • Data file S2 (Microsoft Excel format). DEGs with FDR < 0.05 and FPKM > 10 for R6/2 versus NCAR at 1, 2, and 3 months of age.
  • Data file S3 (Microsoft Excel format). Genes that belong to each of the WGCNA modules identified in fig. S9.
  • Data file S4 (Microsoft Excel format). Shared and unique DEGs between ZFPDelta and ZFP (R6/2 versus NCAR, FDR < 0.05, FPKM > 10).
  • Data file S5 (Microsoft Excel format). Raw replicate values that were used to generate the graphs shown in the figures.