Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Decrease in hepatic Gα13 in patients with diabetes.
  • Fig. S2. Correlations between hepatic Gα13 and lipid profiles in patients with diabetes.
  • Fig. S3. A metabolic and liver function profile in G13 LKO mice fed a high-fat diet.
  • Fig. S4. Impaired glucose homeostasis and insulin resistance in G13 LKO mice fed a normal diet.
  • Fig. S5. Correlations between serum ITIH1 concentrations and lipid profiles in patients with NAFLD.
  • Fig. S6. ITIH1 and ITIH2 concentrations in the liver of patients with diabetes or G13 LKO mice fed a high-fat diet.
  • Fig. S7. Immunoblotting for extracellular matrix and cytoplasmic protein markers.
  • Fig. S8. Immunofluorescence staining for ITIH1-hyaluronan complexes in vitro.
  • Fig. S9. Increase in O-GlcNAC transferase–mediated O-GlcNAcylation and ITIH1 concentrations after loss of Gα13.
  • Fig. S10. LC-MS/MS analysis of O-GlcNAc modification of human ITIH1.
  • Fig. S11. O-GlcNAC transferase–mediated stabilization of ITIH1 through suppression of proteasomal degradation.
  • Fig. S12. The effect of ITIH1 silencing on insulin sensitivity in vitro and effects of anti-ITIH1 antibody treatment of mice in vivo.
  • Fig. S13. The effect of a high to low glucose transition on O-GlcNAcylation and Gα13 concentrations in mice in vivo.
  • Table S1. Characteristics of subjects with NAFLD in cohort #1.
  • References (4251)

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