Supplementary Materials

The PDF file includes:

  • Fig. S1. Fluorescence-based laser microdissection and downstream transcriptomic and bioinformatic analyses.
  • Fig. S2. THP treatment of HK-2 cells results in inhibition of multiple RAC1-dependent pathways.
  • Fig. S3. Quantitation of membrane-associated and cellular RAC1 in kidney sections from THP+/+ and THP−/− mice.
  • Fig. S4. Free radical scavenging proteins are down-regulated in the S3 tubules of THP−/− mice.
  • Fig. S5. Molecules of the RAC1/JNK/c-JUN signaling network involved in free radical synthesis.
  • Fig. S6. ROS concentrations are increased within the S3 proximal tubules of THP−/− mice.
  • Fig. S7. Electron microscopy studies of mitochondrial morphology in S3 segments.
  • Fig. S8. H2O2 activates IL-23 expression in HK-2 cells.
  • Fig. S9. THP plasma concentrations in liver transplant recipients who did not develop AKI.
  • Table S1. Demographic and baseline clinical data for liver transplant surgery group and healthy controls.
  • Legends for data files S1 to S3

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Gene arrays S3.
  • Data file S2 (Microsoft Excel format). Proteomics HK-2 cell THP stimulation.
  • Data file S3 (Microsoft Excel format). Oxidized phospholipidomics from kidneys.