Supplementary Materials

The PDF file includes:

  • Materials and methods
  • Fig. S1. WNT5A and SFRP5 expression in ScAT, IMAs, and PVAT.
  • Fig. S2. Ex vivo effect of WNT5A on NOX isoform gene expression in IMAs.
  • Fig. S3. Association of plasma WNT5A/SFRP5 with arterial NOX expression.
  • Fig. S4. Effects of WNT5A on endothelial function and eNOS coupling.
  • Fig. S5. Phenotyping of the TetO-Wnt5a rtTA mouse model.
  • Fig. S6. Phenotyping of isolated primary VSMCs.
  • Fig. S7. WNT5A dysregulated genes and pathways and effects on VSMC migration.
  • Fig. S8. WNT5A and VSMC phenotypic switch.
  • Fig. S9. USP17 as the maximally up-regulated target in response to WNT5A and USP17 transfection in HeLa cells.
  • Fig. S10. Schematic diagram with proposed mechanism.
  • Table S1. Demographic characteristics of the study participants.
  • Table S2. Demographic characteristics of study 1 participants per BMI group.
  • Table S3. Multivariate linear regression models of calcified plaque progression and new-onset calcification.
  • Table S4. Multivariate linear regression models of arterial NADPH-stimulated superoxide (O2•−) production.
  • References (4247)

[Download PDF]

Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Individual subject-level data.