Supplementary Materials

The PDF file includes:

  • Fig. S1. Anti-HER2/CD3 treatment-induced cytokine release in mice.
  • Fig. S2. Effect of anti-HER2/CD3 treatment on peripheral T cell status in mice.
  • Fig. S3. Characterization of single-cell RNA-seq data.
  • Fig. S4. Single-cell expression analysis of treatment-based expression patterns within human PBMCs.
  • Fig. S5. Effect of pharmacologic or genetic IL-6 signaling intervention on TDB pharmacology.
  • Fig. S6. Effect of prophylactic IL-1 blockade on anti-HER2/CD3 TDB pharmacology.
  • Fig. S7. CD8-specific expression analysis after TDB treatment.
  • Fig. S8. Expression of TNFR1 and TNFR2 in human T cells and monocytes.
  • Fig. S9. Effect of prophylactic TNF-α blockade on anti-HER2/CD3 TDB pharmacology.
  • Fig. S10. Schematic representation of anti-HER2/CD3 TDB–induced cytokine release.

[Download PDF]

Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Marker gene sets used for cell type identification in single-cell RNA-seq data.
  • Data file S2 (Microsoft Excel format). Original data.