Supplementary Materials

The PDF file includes:

  • Fig. S1. Gating strategy used for flow sorting lymphocyte populations in rhesus monkeys.
  • Fig. S2. Logistic regression prediction model in the Ad26/MVA + gp140 arm.
  • Fig. S3. qPCR validates higher GES in B cells as protective.
  • Fig. S4. Correlation of gene expression with magnitude of immune responses in the 09-11 and 13-19 studies.
  • Table S1. Total number of enriched genes in the B cell gene signature for the different trials in this study.
  • Table S2. Time points for different assays in the 09-11 and 13-19 studies.
  • Table S3. B cell gene signature enrichment associated with higher magnitude of immune responses in the 09-11 and 13-19 studies.
  • Table S4. Univariate analysis of immune responses and GES on time to infection in the 13-19 study.
  • Table S5. Univariate analysis of immune responses and GES on time to infection in the 09-11 study.
  • Legends for Data files S1 and S2

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Enriched GSEA gene sets (NES ≥ 1.4, P < 0.001) in different vaccine regimens in the 09-11 and 13-19 studies.
  • Data file S2 (Microsoft Excel format). Functional categorization of the 53 genes in the B cell gene signature by network analysis.