Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Expression of GHSR1α increased in hippocampi from subjects with AD and 9-month-old 5×FAD mice.
  • Fig. S2. Expressions of Ghsr1α and Drd1 were validated in transfected HEK 293T cells.
  • Fig. S3. Schematic diagram shows the sequence of full-length Ghsr1α and its truncating mutants.
  • Fig. S4. FLAG-tagged Ghsr1α and its truncating mutants were similarly expressed in HEK 293T cells.
  • Fig. S5. The interaction between Ghsr1α mutants and Aβ42 was assessed by using Co-IP.
  • Fig. S6. Schematic diagram represents FlAsH-FRET assay for Ghsr1α activity and the impact of oligomeric Aβ42 on Ghsr1α activation.
  • Fig. S7. GHSR1α/DRD1 complex density was negatively correlated with hippocampal soluble Aβ40 or Aβ42 amounts in subjects with AD.
  • Fig. S8. Expression of hippocampal DRD1 remained unaltered in hippocampi from subjects with AD and 5×FAD mice.
  • Fig. S9. Oligomeric Aβ42 did not affect agonist-induced activation of Drd1 or form complex with Drd1.
  • Fig. S10. Input/output curves of fEPSPs were similar in four types of transgenic mice.
  • Fig. S11. Aβ deposition in the hippocampus remained unchanged in Ghsr null/5×FAD mice as compared with their 5×FAD littermates.
  • Fig. S12. Serum ghrelin amounts were similar in four types of transgenic mice.
  • Fig. S13. Loss of Ghsr1α suppressed postsynaptic CaMKII activation in the hippocampus.
  • Fig. S14. The optimal doses of MK0677 and SKF81297 were determined by their augmenting effect on synaptogenesis in cultured hippocampal neurons.
  • Fig. S15. Time course of LTP and fEPSP amplitudes was not changed by different treatments on hippocampal slices from nonTg mice.
  • Fig. S16. The doses of MK0677/SKF81297 treatment were optimized on the basis of the influence on body weight, serum ghrelin, and behavioral performance.
  • Fig. S17. MK0677/SKF81297 [MK0677 (1 mg/kg) and SKF81297 (1.5 mg/kg)] treatment on mice had no effect on hepatic, renal, and hippocampal cell density.
  • Fig. S18. MK0677/SKF81297 [MK0677 (1 mg/kg) and SKF81297 (1.5 mg/kg)] treatment improved neurogenesis in the dentate gyrus of 5×FAD mice.
  • Table S1. Human brain tissue information.
  • References (6472)

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Other Supplementary Material for this manuscript includes the following:

  • Table S2. Raw data (provided as separate Excel file).