Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Flow cytometry gating strategy for detection of anti-HIV CARs on the surface of CD4+ and CD8+ T cells.
  • Fig. S2. Ratio of CD4+ and CD8+ T cells for different donors used in the study.
  • Fig. S3. In vitro killing efficacy of multispecific anti-HIV CAR-T cells against PBMCs infected with an IMC expressing Env from HIV-1 clade B viruses.
  • Fig. S4. Multispecific anti-HIV CAR-T cells exhibit superior in vitro killing efficacy at low E:T ratios.
  • Fig. S5. In vitro killing efficacy of multispecific anti-HIV CAR-T cells against PBMCs infected with an IMC expressing Env from two HIV-1 clade C viruses.
  • Fig. S6. In vitro killing efficacy of multispecific anti-HIV CAR-T cells against PBMCs infected with an IMC expressing Env from HIV-1 clades AC, BC, and G viruses.
  • Fig. S7. In vitro killing efficacy of multispecific anti-HIV CAR-T cells against PBMCs infected with an IMC expressing Env from HIV-1 clade AE virus.
  • Fig. S8. Percentage of HIV-1 inhibition for conventional and multispecific anti-HIV CAR-T cells tested against PBMCs infected with 11 different Env-IMC-LucR viruses encoding genetically diverse env genes.
  • Fig. S9. In vitro elimination by anti-HIV CAR-T cells of PBMCs infected with an IMC expressing Env from HIV-1 clade C virus.
  • Fig. S10. Simultaneous expression of the mD1.22 and m36.4 domains on the surface of mono- and duoCAR-T cells.
  • Fig. S11. Detection of total cell-associated HIV DNA in the spleens of HIV-infected NSG mice treated with mono- and duoCAR-T cells.
  • References (55, 56)

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Other Supplementary Material for this manuscript includes the following: