Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. von Kossa staining of HA-PELGA filaments.
  • Fig. S2. Stress-controlled cyclic thermal mechanical tests of 3D-printed macroporous 25% HA-PELGA(2/1) scaffolds.
  • Fig. S3. DSC traces of as-prepared 25% HA-PELGA scaffolds during heating.
  • Fig. S4. HA-PELGA(8/1) graft preparation without sacrificial PVA.
  • Fig. S5. Weight comparison between HA-PELGA grafts prepared with and without sacrificial PVA.
  • Fig. S6. Pull-out test of in vitro graft fixation.
  • Fig. S7. GPC traces of degradation products of HA-PELGA(8/1) and HA-PELGA(2/1).
  • Fig. S8. Cytocompatibility of degradation products of HA-PELGA grafts.
  • Fig. S9. Experimental design and μCT quantification of HA-PELGA graft-guided bone regeneration.
  • Fig. S10. New bone formation and maturation over time templated by HA-PELGA with or without 400-ng rhBMP-2/7 by polarized light microscopy and trichrome staining.
  • Fig. S11. Experimental design, μCT quantification, and torsion test of HA-PELGA and 400-ng rhBMP-2/7 graft-guided bone regeneration.
  • Fig. S12. Histological micrographs of H&E-, ALP/TRAP-, and Tol blue–stained femoral sections from HA-PELGA(2/1) and 400-ng rhBMP-2/7–treated group.
  • Fig. S13. Representative micrographs of H&E-stained sections of organs.
  • Fig. S14. Healing of 5-mm rat femoral segmental defects by collagen sponge preabsorbed with 400-ng rhBMP-2/7.
  • Table S1. Literature BMP therapeutics applied to rat femoral segmental defects.
  • References (7385)

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Individual subject-level data.