Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. PTEN affects mitochondrial membrane potential and assimilation of metabolites.
  • Fig. S2. HIF1α is not increased in PTEN-null cells.
  • Fig. S3. PTEN regulates succinate secretion during infection with P. aeruginosa.
  • Fig. S4. PTEN is reduced in patients with CFTR mutations.
  • Fig. S5. Succinate in BAL is increased in P. aeruginosa–infected CFTRΔF508/ΔF508 mice.
  • Fig. S6. High succinate induces oxidative stress and metabolic adaptation in P. aeruginosa in LB and CF sputum–like media.
  • Fig. S7. Succinate-stressed WT P. aeruginosa produce larger colonies in succinate-free LB agar plates.
  • Fig. S8. Succinate-stressed P. aeruginosa biofilms are more tolerant to succinate.
  • Fig. S9. The PTEN-succinate axis does not regulate S. aureus adaptation to the airway.
  • Fig. S10. Phenotypic characterization of P. aeruginosa strains recovered over 4 years from the CF airway.
  • Fig. S11. Mucoid and SCV CF isolates have differential preference for succinate, acetate, and L-threonine compared with PAO1.
  • Fig. S12. CF-adapted P. aeruginosa excrete succinate.
  • Fig. S13. Host-adapted P. aeruginosa induce less secretion of proinflammatory cytokines in the airway.
  • Fig. S14. IL-1β induced by metabolically adapted P. aeruginosa isolates is not trapped inside neutrophils.
  • Fig. S15. IRG1 expression by resident alveolar macrophages and neutrophils in P. aeruginosa–infected mice.
  • Fig. S16. Clinical isolates of P. aeruginosa do not induce cell death in IRG1+ BAL monocytes.
  • Table S1. CFTR genotype and P. aeruginosa airway abundance in healthy patients and patients with CF.
  • Table S2. Pathoadaptive mutations conserved in all 17 CF P. aeruginosa.
  • Table S3. Accession numbers for P. aeruginosa isolates.
  • Table S4. PAMPs found mutated in all CF P. aeruginosa isolates.
  • Table S5. Sequences of the primers used for qRT-PCR.

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Raw data from figures.