Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. AVPR1A mRNA is increased and associated with the presence of AR-V7 in metastatic PC.
  • Fig. S2. AVPR1A mRNA is expressed in AR-positive CRPC cell lines.
  • Fig. S3. AVPR1A knockdown has minimal to no antiproliferative effects on cells other than AR-positive CRPC.
  • Fig. S4. Rescue experiments confirm that shRNA-based knockdown specifically targets AVPR1A.
  • Fig. S5. AVP stimulates calcium release in PC cell lines and a prostate epithelial cell line via AVPR1A.
  • Fig. S6. Phosphoproteome profiling reveals that AVP stimulates AVPR1A-mediated ERK and CREB phosphorylation in CRPC cells.
  • Fig. S7. AVPR1A is a signaling effector and drug target for CRPC.
  • Table S1. Top 10 genes regulated by AR-V7 and VAV3 in the CRPC cell line 22Rv1.
  • Table S2. List of shRNA oligonucleotide sequences for pLKO.1 plasmid cloning.

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Other Supplementary Material for this manuscript includes the following: