Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Experimental pipeline used to identify cancer neoepitopes.
  • Fig. S2. Phenotypic characterization of CD8+ TILs from human bone marrow samples.
  • Fig. S3. Generation of aAPCs expressing single patient-specific HLA class I molecules.
  • Fig. S4. Healthy donors exhibit negligible responses against endogenous neoantigens.
  • Fig. S5. Neoepitope tetramers are patient specific and exhibit negligible nonspecific binding.
  • Fig. S6. Phenotypic characterization of neoepitope-specific CD8+ TILs.
  • Fig. S7. Patient-specific transcriptional profiles.
  • Fig. S8. Phenotypic characterization of patient-specific CD19+ tumor cells.
  • Table S1. Patient ALL subtypes.
  • Table S2. FPKM values for HLA genes.
  • Table S3. Patient-specific neoepitopes.
  • Table S4. Tetramers used for specificity assays.
  • Table S5. Fluidigm primer list.
  • Table S6. Peptides for functional assays.
  • Table S7. Mutant allele frequencies for sequenced mutations.
  • References (7181)

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Other Supplementary Material for this manuscript includes the following:

  • Table S8 (Microsoft Excel format). Single-cell indexed fluorescence-activated cell sorting median fluorescence intensity.
  • Data file S1 (Microsoft Excel format). Primary data.