Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Genome equivalent (GE), infectivity and plaque morphology of T164S mutant virus.
  • Fig. S2. Infectivity of wild-type and T164S mutant virus in the mosquito vector A. aegypti.
  • Fig. S3. Ex vivo human PBMC infection with wild-type or T164S mutant virus in the absence or presence of humanized 4G2 antibody.
  • Fig. S4. Heat map expression profile of inflammation-associated genes after infection of human PBMCs with wild-type or T164S mutant virus.
  • Fig. S5. Heat map expression profile of inflammation-associated genes after infection of human PBMCs with wild-type or T164S mutant virus in the presence of humanized 4G2 antibody.
  • Fig. S6. Heat map expression profile of clustered inflammation-associated genes after infection of human PBMCs with wild-type or T164S mutant virus.
  • Fig. S7. Heat map expression profile of clustered inflammation-associated genes after infection of human PBMCs with wild-type or T164S mutant virus in the presence of humanized 4G2 antibody.
  • Fig. S8. Viremia and proinflammatory cytokine production in AG129 mice infected with DENV2 wild-type or T164S mutant virus.
  • Fig. S9. Pairwise growth competition assay between wild-type and T164S mutant virus.
  • Fig. S10. Proposed mechanism of the increased secretion of the T164S NS1 hexamer.
  • Table S1. List of add-on genes in the NanoString Human Inflammation v2 codeset.
  • Table S2. Mass spectrometry parameters used for determining the lipid composition of the NS1.
  • Table S3. Summary of lipid species and their relative abundance extracted from immunoaffinity-purified wild-type or T164S mutant NS1 proteins.

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Individual-level data for characterization of the T164S mutation in NS1 of dengue virus.
  • Data file S2 (Microsoft Excel format). Individual-level data for Infection of human PBMCs ex vivo with wild-type or T164S mutant dengue virus in the absence or presence of humanized 4G2.
  • Data file S3 (Microsoft Excel format). Individual-level data for nonlethal infection of AG129 mice with wild-type or T164S mutant dengue virus.
  • Data file S4 (Microsoft Excel format). Individual-level data for lethal infection of AG129 mice with wild-type or T164S mutant dengue virus.