Supplementary Materials

This PDF file includes:

• Fig. S1. In vivo acoustic resolution PAFC clinical prototype.
• Fig. S2. Optical schematics for delivery of laser radiation to skin.
• Fig. S3. Optimization of laser parameters in the PAFC setup for detection of CTCs.
• Fig. S4. Typical examples of PA signal amplitude and shape from vessels and artifacts.
• Fig. S5. Examples of small CBCs observed in a few healthy volunteers.
• Fig. S6. PA traces from hand veins of patients with melanoma and PA signal rates.
• Fig. S7. In vitro PA testing of blood samples from healthy volunteers and patients with melanoma.
• Fig. S8. Examples of PA traces in blood samples from a healthy volunteer and six patients with melanoma in vitro.
• Fig. S9. Melanin-induced quenching of fluorescence in melanoma CTCs.
• Fig. S10. In vitro conventional flow cytometry of blood samples from patients with melanoma.
• Fig. S11. Melanoma cell detection in patients’ blood samples after magnetic enrichment in vitro.
• Fig. S12. Visualization of cells using staining procedures.
• Fig. S13. Immunocytochemical analysis of blood samples from patients with melanoma.
• Fig. S14. Sensitivity of qRT-PCR for detecting melanoma markers in healthy blood sample spiked with A2058 human melanoma cells.
• Table S1. Patient parameters.
• Table S2. Detection of CTCs in melanoma patients with PAFC in vitro.
• Table S3. Detection of CBCs in melanoma patients with PAFC in vitro.
• Table S4. Results of testing the blood samples from patients with melanoma by flow cytometry in vitro.
• Table S5. Relative gene expression profile of melanoma markers in patients with melanoma.
• Table S6. Comparison of data for selected patients in vivo with the results ex vivo using different in vitro assays.

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